Soluble ACE2 (Human) His Tagged (HEK293) at 20 ug per vial.
Tag: His Tag on its C-terminus
Expression: HEK293 cells animal free culture
Purity:> 95%
MW: 110-120 KD (Due to Glycoylation)
Tested by anti His Tagged Protein Monoclonal Antibody on RBD-S1 Spike Protein pre-coated microplates.
Tested by Biotinylated RBD His Tag and Streptavidin-HRP on human ACE2 His Tag Pre-coated Microplates.
The enzyme specific activity to cleave a fluorogenic peptide substrate, McaYVADAPK (Dnp)OH, is >1000 pmols/min/μg.
Endotoxin<1.0 EU per μg of the protein as determined by the LAL method
References:
Gheblawi M., et al. Angiotensin-Converting Enzyme 2: SARS-CoV-2 Receptor and Regulator of the Renin-Angiotensin System. Circulation Research. 2020;126:1456–1474
Features
Biological activity test: EC50= 0.235 nM/L ~1.05 nM/L Specific Binding
to the recombinant S-RBD of SARS-CoV-2 (2019-nCoV) coated microplates
(200ng/well)Protein ID: NP_068576.1
The enzyme specific activity to cleave a fluorogenic peptide substrate, McaYVADAPK (Dnp)OH, is >1000 pmols/min/μg.
Gene ID: NM_021804.2
Sequence: Human ACE2 (18-740) extracellular domain.
Tag: His Tag on its C-Terminus
Expression: HEK293 animal free culture
MW: 110-120 KD
Purity: >95%
Endotoxin:< 1.0 EU per μg protein as determined by the LAL method.