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Angiotensin-Converting Enzyme 2 (ACE2) (Human) (HEK293)
Angiotensin-Converting Enzyme 2 (ACE2) (Human) Biotinylated
 


Product Code: 00707-01-1000
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Description Technical Specs
 
Feature:
  • Soluble ACE2 (Human) His Tagged (HEK293) at 20 ug per vial.
  • Tag: His Tag on its C-terminus
  • Expression: HEK293 cells animal free culture
  • Purity:> 95%
  • MW: 110-120 KD (Due to Glycoylation)
  • Tested by anti His Tagged Protein Monoclonal Antibody on RBD-S1 Spike Protein pre-coated microplates.
  • Tested by Biotinylated RBD His Tag and Streptavidin-HRP on human ACE2 His Tag Pre-coated Microplates.
  • The enzyme specific activity to cleave a fluorogenic peptide substrate, McaYVADAPK (Dnp)OH,
    is >1000 pmols/min/μg.
  • Endotoxin<1.0 EU per μg of the protein as determined by the LAL method
References:
  • Gheblawi M., et al. Angiotensin-Converting Enzyme 2: SARS-CoV-2 Receptor and Regulator of the Renin-Angiotensin System. Circulation Research. 2020;126:1456–1474
Features
    • Biological activity test: EC50= 0.235 nM/L ~1.05 nM/L Specific Binding
    • to the recombinant S-RBD of SARS-CoV-2 (2019-nCoV) coated microplates
    • (200ng/well)Protein ID: NP_068576.1
    • The enzyme specific activity to cleave a fluorogenic peptide substrate, McaYVADAPK (Dnp)OH,
      is >1000 pmols/min/μg.
    • Gene ID: NM_021804.2
    • Sequence: Human ACE2 (18-740) extracellular domain.
    • Tag: His Tag on its C-Terminus
    • Expression: HEK293 animal free culture
    • MW: 110-120 KD
    • Purity: >95%
    • Endotoxin:< 1.0 EU per μg protein as determined by the LAL method.


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